Rudolf Buchheim (1 March 1820 – 25 December 1879) was a German pharmacologist who was born in Bautzen. In 1845 he earned his doctorate from the University of Leipzig and soon after became a professor at the University of Dorpat. While at Dorpat he created the first pharmacological institute at that school. In 1867 he became professor of pharmacology and toxicology at the University of Giessen.
Buchheim is remembered for his pioneer work in experimental pharmacology. He was instrumental in turning pharmacology from an empirical study of medicine into an exact science. He introduced the bioassay to pharmacology, and created a methodology for determining the quantative and medical aspects of chemical substances.
While a student in Leipzig, Buchheim translated Jonathan Pereira's (1804–1853) handbook of pharmacology from English into German. He also edited the book, eliminating obsolete and ineffectual medicines and practices, while adding updated information, including a chapter of his own called Art der Wirkung (The Pharmacological Action). A well-known student of his was chemist Oswald Schmiedeberg (1838–1921), who was to become the "founder of modern pharmacology". Today at the University of Giessen is the Rudolf Buchheim Institute for Pharmacology.